Putting firefly tails to work in water treatment (via the Telegraph Journal)Visit TelegraphJournal.com for full article and video Jenny Keleher November 10, 2015
ILLUMINATING WATER TREATMENT in the lab
Fredericton’s LuminUltra Technologies Ltd. saw a gap in waste water treatment testing, and filled it by co-opting the firefly’s glow.
Living things use a molecule called adenosine triphosphate – ATP – to store energy. It’s energy“cash”the organism can spend on whatever activity it needs to do.
For a firefly, bursts of light – a phenomenon called“bioluminescence”– are the best way to spend at least a portion of the ATP it generates and stores from the energy it gets from its food. The winged beetle’s blinking back end, which works to ward off predators and attract mates, requires a finely tuned chemical system.
That chemical system is what LuminUltra has put to work.
From tail to test tube
Jeremy Duguay is a senior account manager with LuminUltra. He’s primarily on the company’s sales and support side, but with a PhD from Queen’s in Kingston, Ont., he’s well-placed to explain how the science works.
“It’s the same thing as a firefly tail. You see it light up at night – that is the ATP molecule reacting with the luciferase enzyme and the luciferin substrate,”says Duguay.
The molecules take their titles from Lucifer, whose name means“bringer of light,”or“morning star.”But for LuminUltra, it’s less about fallen angels, and more about finding microscopic bugs in water.
“We’ve taken that reaction out of the firefly tail, put it into a test tube, essentially,”says Duguay.“Once you have the ATP extracted from your sample, you mix it with some very specific chemistries, with the luciferin and luciferase in there, then quantify the light in the luminometer – that’s the piece of equipment that we also manufacture. Once you have the amount of light, it’s directly proportional readout of how much biological activity there is in a sample.”
It’s a relatively simple formula: Living cells use ATP, and if you can measure it – in this case by using a luminometer to measure the light produced by the luciferase reaction, which relies on ATP – you can correlate the brightness of your test tube to the amount of living microbes in your sample.
Unlike Lucifer, microbes are neither good nor bad by nature. For humans, however, microbiological activity can be anywhere from essential to devastatingly harmful.
LuminUltra, which officially launched in 2003 and now employs about 30, first targeted customers who were looking to understand one“essential”role of microbes: as waste water cleanup crews. By measuring how much ATP you have in your water, you can find out whether the bugs are healthy.
“It all started with the first test, and the concept the company was designed for: monitoring the biomass in biological waste water treatment,”says Duguay. “That is a process that takes micro-organisms, utilizes them to break down waste and produces clean water on the back end of the plant.
“The biomass is the most important part – the driving engine of that process. Yet there were very few tools people had available in that application to actually monitor and see what the biomass was doing. That void in the marketplace is what set about LuminUltra’s first technology to monitor and quantify that.”
Healthy bugs aren’t always what you want
The company quickly realized that measuring ATP in water samples had applications beyond waste water treatment – in the areas where microbes weren’t so welcome. The concept of an ATP test isn’t new, but LuminUltra has tuned it for specific applications.
In a process such as distributing clean drinking water to a city, the microbes are the enemy. And to effectively combat the enemy, you need to act fast.
“The tools that were available to monitor micro-organisms in those applications were traditional tools: things like a microscope and culture-based tests, which are gold standards and very good at what they do, they just take a fair bit of time and expertise.
“Particularly the time point is a big issue if you’re taking samples from a drinking water system and testing them for micro-organisms, and it takes two days or more to get results back. During that time, you could still have a problem there, but you don’t know about it till the results come back.”
What the test kits lose in terms of identifying the specific type of micro-organism in a sample, they gain in speed and portability. Duguay says the kits fit in a briefcase, and give results in anywhere from three to seven minutes. He calls LuminUltra’s kits a “first line of defence.”
Customers could include drinking water treatment plants and manufacturing interests – both spots where microbes can foul the final product. squishing fireflies Micro-organisms may be everywhere, and the ATP they create, spend, and recreate may also be ubiquitous, but what about the luciferase and luciferin – does every test require the squishing of five or 10 light-bringing beetles? “They did have to have graduate students or poor undergrads going out, collecting and actually harvesting the enzyme from the fireflies at the time, and purifying it,”says Duguay.
“It’s commercially available now from recombinant technology.”
Recombinant technology is a way of splicing DNA segments and using microbiological “factories” to mass-produce a desired substance. Duguay can honestly tell a customer that no fireflies were harmed in the making of this test kit. The kit, in fact, has potential to prevent harm in the human context.
“Go to a city like New York or Boston – there’s a lot of pipes under the city. The water gets treated, gets pushed out under the city for people to drink. A lot can happen between the time it leaves the treatment plant and the time it comes to your tap. Being able to actually go out in the field, and test different locations, for water quality, very rapidly, is a huge advantage for a municipality that needs to take care of the water that people are consuming. If they spot a problem, they can take action right away.”
Why new Brunswick?
Duguay, a native of Sussex, is happy to put his microbiology know-how to work in his home province. The company, headquartered on King Street in downtown Fredericton, has customers spanning this continent and others. “We’re excited about the opportunity to be back home,”he says.“The opportunities to talk about science in New Brunswick are few and far between. It’s nice to be able to talk about that now.”
Science and research columnist, and copy editor at Brunswick News.
In the lab appears every other week in innovate